Worm Research Lab

McMaster University

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orientation

Gupta Lab Orientation Information

Training/safety-related (before starting experiments in the lab!)

  • Complete the safety training (available through online Mosaic system). WHMIS is mandatory for everyone. Update the Safety Training checklist. A copy of signed checklist must be filed in the lab binder.
  • Fill out the “Working alone” and Volunteer agreement forms, if required.
  • Familiarize with equipment in the lab, read SOPs and update the Equipment Training checklist. It is important for your safety and keeping equipment in good, working condition.
  • Read and sign the Gupta Lab Safety Procedures form. File it in the lab binder.

General

  • Familiarize yourself with various chemical stocks (liquid/solid) stored at room temperature and in -4C refrigerator and -20C freezer.
  • Learn about enzymes, kits, and other molecular biology stocks in lab freezers.
  • Learn to prepare common media and buffers (LB, M9, PO4 etc.).
  • Keep your lab notebook up-to-date. Discuss with Bhagwati if you need access to online lab database resources and LabArchives digital notebook systems. All experiment-related data should be stored in LabArchives.

Worm-related

  • Read basic worm culturing techniques and C. elegans genetics and anatomy (“Methods” chapter in Wood's C. elegans book and the websites www.wormatlas.org and www.wormbook.org)
  • Review basic C. elegans literature. A few important links are posted in the Resources section.
  • Using Nomarski microscope examine different stages of the developing vulva and gonad in hermaphrodites and take pictures
  • Learn to
    • Make and seed C. elegans culture plates (NG-Agar)
    • Make platinum wire pick for handling worms
    • Propagate worm cultures by bulk transfer
    • Transfer single/few worm(s) without poking holes in the agar
    • Recognize males and hermaphrodites
    • Distinguish between egg, larval, and adult stages
    • Recognize L4 larval stage hermaphrodites (based on vulval invagination)
    • Recognize fresh young hermaphrodites (empty uterus, no fertilized eggs) and gravid hermaphrodites
    • Mount worms on glass slides for Nomarski observations
  • Learn to setup a simple genetic cross
    • Cross Unc/Dpy hermaphrodites to wildtype males
    • Clone 3 wildtype-looking worms in the F1 generation
    • Recover the homozygous mutant in F2 generation

Microscopy-related

  • Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling.
  • Cover the Nomarski microscopes after every use. Microscope lenses are expensive and dust can cause damage.
  • Organize captured images in a folder on the computer. Label files and folders clearly so these could be easily identified. Unorganized files may get deleted without prior warning!
  • Use the Log Book. It is a MUST for everyone. Also, keep an eye on others to make sure that they are doing it as well.
  • Report problems with microscopes to supervisor PROMPTLY. It is a collective responsibility of lab workers to keep things running smoothly.


BE A RESPONSIBLE PERSON! DO NOT MISUSE EQUIPMENT/SUPPLIES AND REPORT PROBLEMS PROMPTLY.

NO FOOD OR DRINK IS ALLOWED IN THE LAB!

orientation.txt · Last modified: 2018/05/09 15:38 by admin