Worm Research Lab

McMaster University

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orientation

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Gupta Lab Orientation Information

Training/safety-related (before starting experiments in the lab!)

  • Complete all safety trainings available through Mosaic online system. WHMIS is mandatory for everyone. Update the Safety Training checklist. A copy of signed checklist must be filed in the lab binder.
  • Fill out the “Working alone” and Volunteer agreement forms, if required.
  • Familiarize with various equipment in the lab, read SOPs and update the Equipment Training checklist. It is important for your safety and keeping equipment in good working condition.
  • Read and sign the Gupta Lab Safety Procedures form. File it in the lab binder.

General

  • Familiarize yourself where equipment and other general things are kept in the lab.
  • Familiarize yourself with various chemicals (liquid and solid) in the lab.
  • Keep your lab notebook up-to-date. Discuss with Bhagwati if you need access to online LabArchives digital notebook system. All lab data are stored in LabArchives.

Worm-related

  • Read basic worm culturing techniques and C. elegans genetics and anatomy (“Methods” chapter in Wood's C. elegans book and the websites www.wormatlas.org and www.wormbook.org)
  • Review basic C. elegans literature. A few important links are posted in the Resources section.
  • Using Nomarski microscope examine different stages of the developing vulva and gonad in hermaphrodites and take pictures
  • Learn to
    • Make and seed C. elegans culture plates (NG agar)
    • Make platinum wire pick for handling worms
    • Propagate worm cultures by bulk transfer
    • Transfer single/few worm(s) without poking holes in agar
    • Recognize males and hermaphrodites
    • Distinguish between egg, larval and adult stages
    • Recognize L4 stage hermaphrodites (based on the vulval invagination)
    • Recognize young hermaphrodites (without fertilized eggs in the uterus) and those that are actively laying eggs
    • Mount worms on glass slides for Nomarski observations
  • Learn to setup a simple genetic cross
    • Cross Unc/Dpy hermaphrodites to wild type males
    • Clone 3 wild type-looking worms in the F1 generation
    • Recover the mutant in F2 generation

Microscopy-related

  • Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling.
  • Cover the Nomarski microscopes EVERYTIME after you have finished using it. Microscope lenses are expensive.
  • Organize your imaging files on the computer. Create your own folder to keep all the data files inside. Unorganized files may get deleted without any warning!
  • Use the Log Book. It is a MUST for everyone. You should also keep an eye on others to make sure that they are doing it as well.
  • Report problems with microscopes to me IMMEDIATELY. It is a collective responsibility of lab workers to keep things running smoothly.


BE A RESPONSIBLE PERSON! DO NOT MISUSE EQUIPMENT/SUPPLIES AND REPORT PROBLEMS PROMPTLY.

NO FOOD OR DRINK IS ALLOWED IN THE LAB!

orientation.1508850067.txt.gz · Last modified: 2017/10/24 13:01 by 127.0.0.1