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===== Gupta Lab Orientation Information ===== | ===== Gupta Lab Orientation Information ===== | ||
- | **Training/ | + | **Training/ |
- | * Complete | + | * Complete |
- | * Fill out the “Working alone” and <color blue> | + | * Fill out the “Working alone” and <color blue> |
- | * Familiarize with various | + | * Familiarize with equipment in the lab, read SOPs and update the <color blue> |
- | * Read and sign the <color blue> | + | * Read and sign the <color blue> |
**General** | **General** | ||
- | * Familiarize yourself | + | * Familiarize yourself |
- | * Familiarize yourself with various chemicals (liquid | + | * Learn about enzymes, kits, and other molecular biology stocks |
- | * Keep your lab notebook up-to-date | + | * Learn to prepare common media and buffers (LB, M9, PO4 etc.). |
+ | * Keep your lab notebook up-to-date. Discuss with Bhagwati if you need access to online [[https:// | ||
**Worm-related** | **Worm-related** | ||
* Read basic worm culturing techniques and //C. elegans// genetics and anatomy (" | * Read basic worm culturing techniques and //C. elegans// genetics and anatomy (" | ||
+ | * Review basic //C. elegans// literature. A few important links are posted in the [[resources|Resources]] section. | ||
+ | * Using Nomarski microscope examine different stages of the developing vulva and gonad in hermaphrodites and take pictures | ||
+ | |||
* Learn to | * Learn to | ||
- | * Make and seed //C. elegans// culture plates (NG agar) | + | * Make and seed //C. elegans// culture plates (NG-Agar) |
* Make platinum wire pick for handling worms | * Make platinum wire pick for handling worms | ||
* Propagate worm cultures by bulk transfer | * Propagate worm cultures by bulk transfer | ||
- | * Transfer single/few worm(s) without poking holes in agar | + | * Transfer single/few worm(s) without poking holes in the agar |
* Recognize males and hermaphrodites | * Recognize males and hermaphrodites | ||
- | * Distinguish between egg, larval and adult stages | + | * Distinguish between egg, larval, and adult stages |
- | * Recognize L4 stage hermaphrodites (based on the vulval invagination) | + | * Recognize L4 larval |
- | * Recognize young hermaphrodites (without | + | * Recognize |
* Mount worms on glass slides for Nomarski observations | * Mount worms on glass slides for Nomarski observations | ||
- | * Study various stages of the developing vulva and gonad in wild type worms and take pictures | + | |
- | * Setup a genetic cross | + | * Learn to setup a simple |
- | * Cross Unc/Dpy hermaphrodites to wild type males | + | * Cross Unc/Dpy hermaphrodites to wildtype |
- | * Clone 3 wild type-looking worms in the F1 generation | + | * Clone 3 wildtype-looking worms in the F1 generation |
- | * Recover the mutant in F2 generation | + | * Recover the homozygous |
**Microscopy-related** | **Microscopy-related** | ||
* Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling. | * Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling. | ||
- | * Cover the Nomarski microscopes | + | * Cover the Nomarski microscopes after every use. Microscope lenses are expensive |
- | * Organize | + | * Organize |
- | * Use the Log Book. It is a MUST for everyone. | + | * Use the Log Book. It is a MUST for everyone. |
- | * Report problems with microscopes to me IMMEDIATELY. It is a collective responsibility of lab workers to keep things running smoothly. | + | * Report problems with microscopes to supervisor PROMPTLY. It is a collective responsibility of lab workers to keep things running smoothly. |
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