Worm Research Lab

McMaster University

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 ===== Gupta Lab Orientation Information ===== ===== Gupta Lab Orientation Information =====
  
-**Training/​safety-related (before starting ​any experiment ​in the lab!)** +**Training/​safety-related (before starting ​experiments ​in the lab!)** 
-  * Complete ​all safety ​trainings and update ​the <color blue>​Safety Training</​color>​ checklist  +  * Complete ​the safety ​training (available through online Mosaic system). WHMIS is mandatory for everyone. Update ​the <color blue>​Safety Training</​color>​ checklist. A copy of signed checklist must be filed in the lab binder.  ​ 
-  * Fill out the “Working alone” and <color blue>​Volunteer agreement</​color>​ forms, if required  +  * Fill out the “Working alone” and <color blue>​Volunteer agreement</​color>​ forms, if required 
-  * Familiarize with various ​equipment in the lab, read SOPs and update the <color blue>​Equipment Training</​color>​ checklist  +  * Familiarize with equipment in the lab, read SOPs and update the <color blue>​Equipment Training</​color>​ checklist. It is important for your safety and keeping equipment in good, working condition. ​ 
-  * Read and sign the <color blue>​Gupta Lab Safety Procedures</​color>​ form+  * Read and sign the <color blue>​Gupta Lab Safety Procedures</​color>​ form. File it in the lab binder.
  
  
 **General** **General**
-  * Familiarize yourself ​where equipment ​and other general things are kept in the lab +  * Familiarize yourself ​with various chemical stocks (liquid/​solid) stored at room temperature and in -4C refrigerator and -20C freezer. 
-  * Familiarize yourself with various chemicals (liquid ​and solidin the lab +  * Learn about enzymes, kits, and other molecular biology stocks ​in lab freezers. ​ 
-  * Keep your lab notebook up-to-date ​+  * Learn to prepare common media and buffers (LB, M9, PO4 etc.). 
 +  * Keep your lab notebook up-to-date. Discuss with Bhagwati if you need access to online [[https://​www.macwormlab.net/​labdb/​|lab database resources]] and [[http://​www.labarchives.com|LabArchives]] digital notebook systems. All experiment-related data should be stored in LabArchives. ​
  
  
 **Worm-related** **Worm-related**
   * Read basic worm culturing techniques and //C. elegans// genetics and anatomy ("​Methods"​ chapter in Wood's //C. elegans// book and the websites www.wormatlas.org and www.wormbook.org)   * Read basic worm culturing techniques and //C. elegans// genetics and anatomy ("​Methods"​ chapter in Wood's //C. elegans// book and the websites www.wormatlas.org and www.wormbook.org)
 +  * Review basic //C. elegans// literature. A few important links are posted in the [[resources|Resources]] section.
 +  * Using Nomarski microscope examine different stages of the developing vulva and gonad in hermaphrodites and take pictures
 +
   * Learn to    * Learn to 
-    * Make and seed //C. elegans// culture plates (NG agar+    * Make and seed //C. elegans// culture plates (NG-Agar
     * Make platinum wire pick for handling worms      * Make platinum wire pick for handling worms 
     * Propagate worm cultures by bulk transfer ​     * Propagate worm cultures by bulk transfer ​
-    * Transfer single/few worm(s) without poking holes in agar+    * Transfer single/few worm(s) without poking holes in the agar
     * Recognize males and hermaphrodites     * Recognize males and hermaphrodites
-    * Distinguish between egg, larval and adult stages +    * Distinguish between egg, larvaland adult stages 
-    * Recognize L4 stage hermaphrodites (based on the vulval invagination) +    * Recognize L4 larval ​stage hermaphrodites (based on vulval invagination) 
-    * Recognize young hermaphrodites (without ​fertilized eggs in the uterus) and those that are actively laying eggs +    * Recognize ​fresh young hermaphrodites (empty uterus, no fertilized eggs) and gravid hermaphrodites  ​
     * Mount worms on glass slides for Nomarski observations     * Mount worms on glass slides for Nomarski observations
-  * Study various stages of the developing vulva and gonad in wild type worms and take pictures + 
-  * Setup a genetic cross  +  * Learn to setup simple ​genetic cross  
-    * Cross Unc/Dpy hermaphrodites to wild type males  +    * Cross Unc/Dpy hermaphrodites to wildtype ​males  
-    * Clone 3 wild type-looking worms in the F1 generation  +    * Clone 3 wildtype-looking worms in the F1 generation  
-    * Recover the mutant in F2 generation+    * Recover the homozygous ​mutant in F2 generation
  
 **Microscopy-related** **Microscopy-related**
   * Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling. ​   * Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling. ​
-  * Cover the Nomarski microscopes ​EVERYTIME ​after you have finished using it. Microscope lenses are expensive.  +  * Cover the Nomarski microscopes after every use. Microscope lenses are expensive ​and dust can cause damage.  
-  * Organize ​your imaging files on the computer. ​Create your own folder to keep all the data files inside. Unorganized files may get deleted without ​any warning!  +  * Organize ​captured images in a folder ​on the computer. ​Label files and folders clearly so these could be easily identified. Unorganized files may get deleted without ​prior warning!  
-  * Use the Log Book. It is a MUST for everyone. ​You should also keep an eye on others to make sure that they are doing it as well.  +  * Use the Log Book. It is a MUST for everyone. ​Also, keep an eye on others to make sure that they are doing it as well.  
-  * Report problems with microscopes to me IMMEDIATELY. It is a collective responsibility of lab workers to keep things running smoothly. ​+  * Report problems with microscopes to supervisor PROMPTLY. It is a collective responsibility of lab workers to keep things running smoothly. ​
  
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orientation.1464747972.txt.gz · Last modified: 2017/10/24 12:25 (external edit)