Worm Research Lab

McMaster University

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orientation

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Gupta Lab Orientation Information

Training/safety-related (before starting any experiment in the lab!)

  • Complete all safety trainings and update the Safety Training checklist
  • Fill out the “Working alone” and Volunteer agreement forms, if required
  • Familiarize with various equipment in the lab, read SOPs and update the Equipment Training checklist
  • Read and sign the Gupta Lab Safety Procedures form

General

  • Familiarize yourself where equipment and other general things are kept in the lab
  • Familiarize yourself with various chemicals (liquid and solid) in the lab
  • Keep your lab notebook up-to-date

Worm-related

  • Read basic worm culturing techniques and C. elegans genetics and anatomy (“Methods” chapter in Wood's C. elegans book and the websites www.wormatlas.org and www.wormbook.org)
  • Learn to
    • Make and seed C. elegans culture plates (NG agar)
    • Make platinum wire pick for handling worms
    • Propagate worm cultures by bulk transfer
    • Transfer single/few worm(s) without poking holes in agar
    • Recognize males and hermaphrodites
    • Distinguish between egg, larval and adult stages
    • Recognize L4 stage hermaphrodites (based on the vulval invagination)
    • Recognize young hermaphrodites (without fertilized eggs in the uterus) and those that are actively laying eggs
    • Mount worms on glass slides for Nomarski observations
  • Study various stages of the developing vulva and gonad in wild type worms and take pictures
  • Setup a genetic cross
    • Cross Unc/Dpy hermaphrodites to wild type males
    • Clone 3 wild type-looking worms in the F1 generation
    • Recover the mutant in F2 generation

Microscopy-related

  • Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling.
  • Cover the Nomarski microscopes EVERYTIME after you have finished using it. Microscope lenses are expensive.
  • Organize your imaging files on the computer. Create your own folder to keep all the data files inside. Unorganized files may get deleted without any warning!
  • Use the Log Book. It is a MUST for everyone. You should also keep an eye on others to make sure that they are doing it as well.
  • Report problems with microscopes to me IMMEDIATELY. It is a collective responsibility of lab workers to keep things running smoothly.


BE A RESPONSIBLE PERSON! DO NOT MISUSE EQUIPMENT/SUPPLIES AND REPORT PROBLEMS PROMPTLY.

NO FOOD OR DRINK IS ALLOWED IN THE LAB!

orientation.1464747972.txt.gz · Last modified: 2017/10/24 12:25 (external edit)