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 ===== Gupta Lab Orientation Information ===== ===== Gupta Lab Orientation Information =====
  
-**Training/safety-related (before starting any experiment in the lab!)** +**Training/safety-related (before starting experiments in the lab!)** 
-  * Complete all safety trainings and update the <color blue>Safety Training</color> checklist  +  * Complete all safety trainings available through Mosaic online system. WHMIS is mandatory for everyone. Update the <color blue>Safety Training</color> checklist. A copy of signed checklist must be filed in the lab binder.   
-  * Fill out the “Working alone” and <color blue>Volunteer agreement</color> forms, if required  +  * Fill out the “Working alone” and <color blue>Volunteer agreement</color> forms, if required.  
-  * Familiarize with various equipment in the lab, read SOPs and update the <color blue>Equipment Training</color> checklist  +  * Familiarize with various equipment in the lab, read SOPs and update the <color blue>Equipment Training</color> checklist. It is important for your safety and keeping equipment in good working condition.  
-  * Read and sign the <color blue>Gupta Lab Safety Procedures</color> form+  * Read and sign the <color blue>Gupta Lab Safety Procedures</color> form. File it in the lab binder.
  
  
 **General** **General**
-  * Familiarize yourself where equipment and other general things are kept in the lab +  * Familiarize yourself where equipment and other general things are kept in the lab. 
-  * Familiarize yourself with various chemicals (liquid and solid) in the lab +  * Familiarize yourself with various chemicals (liquid and solid) in the lab. 
-  * Keep your lab notebook up-to-date +  * Keep your lab notebook up-to-date. Discuss with Bhagwati if you need access to online [[http://www.labarchives.com|LabArchives]] digital notebook system. All lab data are stored in LabArchives. 
  
  
 **Worm-related** **Worm-related**
   * Read basic worm culturing techniques and //C. elegans// genetics and anatomy ("Methods" chapter in Wood's //C. elegans// book and the websites www.wormatlas.org and www.wormbook.org)   * Read basic worm culturing techniques and //C. elegans// genetics and anatomy ("Methods" chapter in Wood's //C. elegans// book and the websites www.wormatlas.org and www.wormbook.org)
 +  * Review basic //C. elegans// literature. A few important links are posted in the [[resources|Resources]] section.
 +  * Using Nomarski microscope examine different stages of the developing vulva and gonad in hermaphrodites and take pictures
 +
   * Learn to    * Learn to 
     * Make and seed //C. elegans// culture plates (NG agar)      * Make and seed //C. elegans// culture plates (NG agar) 
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     * Recognize young hermaphrodites (without fertilized eggs in the uterus) and those that are actively laying eggs      * Recognize young hermaphrodites (without fertilized eggs in the uterus) and those that are actively laying eggs 
     * Mount worms on glass slides for Nomarski observations     * Mount worms on glass slides for Nomarski observations
-  * Study various stages of the developing vulva and gonad in wild type worms and take pictures + 
-  * Setup a genetic cross +  * Learn to setup a simple genetic cross 
     * Cross Unc/Dpy hermaphrodites to wild type males      * Cross Unc/Dpy hermaphrodites to wild type males 
     * Clone 3 wild type-looking worms in the F1 generation      * Clone 3 wild type-looking worms in the F1 generation 
  • Last modified: 2022/01/07 20:46