Worm Research Lab

McMaster University

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orientation [2016/06/01 02:26]
admin created
orientation [2017/10/24 13:01]
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 ===== Gupta Lab Orientation Information ===== ===== Gupta Lab Orientation Information =====
  
-**Training/​safety-related (before starting ​any experiment ​in the lab!)** +**Training/​safety-related (before starting ​experiments ​in the lab!)** 
-  * Complete all safety trainings ​and update ​the <color blue>​Safety Training</​color>​ checklist  +  * Complete all safety trainings ​available through Mosaic online system. WHMIS is mandatory for everyone. Update ​the <color blue>​Safety Training</​color>​ checklist. A copy of signed checklist must be filed in the lab binder.  ​ 
-  * Fill out the “Working alone” and <color blue>​Volunteer agreement</​color>​ forms, if required  +  * Fill out the “Working alone” and <color blue>​Volunteer agreement</​color>​ forms, if required 
-  * Familiarize with various equipment in the lab, read SOPs and update the <color blue>​Equipment Training</​color>​ checklist  +  * Familiarize with various equipment in the lab, read SOPs and update the <color blue>​Equipment Training</​color>​ checklist. It is important for your safety and keeping equipment in good working condition. ​ 
-  * Read and sign the <color blue>​Gupta Lab Safety Procedures</​color>​ form+  * Read and sign the <color blue>​Gupta Lab Safety Procedures</​color>​ form. File it in the lab binder.
  
  
 **General** **General**
-  * Familiarize yourself where equipment and other general things are kept in the lab +  * Familiarize yourself where equipment and other general things are kept in the lab. 
-  * Familiarize yourself with various chemicals (liquid and solid) in the lab +  * Familiarize yourself with various chemicals (liquid and solid) in the lab. 
-  * Keep your lab notebook up-to-date ​+  * Keep your lab notebook up-to-date. Discuss with Bhagwati if you need access to online [[http://​www.labarchives.com|LabArchives]] digital notebook system. All lab data are stored in LabArchives. ​
  
  
 **Worm-related** **Worm-related**
   * Read basic worm culturing techniques and //C. elegans// genetics and anatomy ("​Methods"​ chapter in Wood's //C. elegans// book and the websites www.wormatlas.org and www.wormbook.org)   * Read basic worm culturing techniques and //C. elegans// genetics and anatomy ("​Methods"​ chapter in Wood's //C. elegans// book and the websites www.wormatlas.org and www.wormbook.org)
 +  * Review basic //C. elegans// literature. A few important links are posted in the [[resources|Resources]] section.
 +  * Using Nomarski microscope examine different stages of the developing vulva and gonad in hermaphrodites and take pictures
 +
   * Learn to    * Learn to 
     * Make and seed //C. elegans// culture plates (NG agar)      * Make and seed //C. elegans// culture plates (NG agar) 
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     * Recognize young hermaphrodites (without fertilized eggs in the uterus) and those that are actively laying eggs      * Recognize young hermaphrodites (without fertilized eggs in the uterus) and those that are actively laying eggs 
     * Mount worms on glass slides for Nomarski observations     * Mount worms on glass slides for Nomarski observations
-  * Study various stages of the developing vulva and gonad in wild type worms and take pictures + 
-  * Setup a genetic cross +  * Learn to setup simple ​genetic cross 
     * Cross Unc/Dpy hermaphrodites to wild type males      * Cross Unc/Dpy hermaphrodites to wild type males 
     * Clone 3 wild type-looking worms in the F1 generation ​     * Clone 3 wild type-looking worms in the F1 generation ​
orientation.txt · Last modified: 2018/05/09 15:38 by admin