Training/safety-related (before starting experiments in the lab!)
Complete the safety training (available through online Mosaic system). WHMIS is mandatory for everyone. Update the Safety Training checklist. A copy of signed checklist must be filed in the lab binder.
Fill out the “Working alone” and Volunteer agreement forms, if required.
Familiarize with equipment in the lab, read SOPs and update the Equipment Training checklist. It is important for your safety and keeping equipment in good, working condition.
Read and sign the Gupta Lab Safety Procedures form. File it in the lab binder.
Familiarize yourself with various chemical stocks (liquid/solid) stored at room temperature and in -4C refrigerator and -20C freezer.
Learn about enzymes, kits, and other molecular biology stocks in lab freezers.
Learn to prepare common media and buffers (LB, M9, PO4 etc.).
Keep your lab notebook up-to-date. Discuss with Bhagwati if you need access to online lab database resources and LabArchives digital notebook systems. All experiment-related data should be stored in LabArchives.
Read basic worm culturing techniques and C. elegans genetics and anatomy (“Methods” chapter in Wood's C. elegans book and the websites www.wormatlas.org and www.wormbook.org)
Review basic C. elegans literature. A few important links are posted in the Resources section.
Using Nomarski microscope examine different stages of the developing vulva and gonad in hermaphrodites and take pictures
Make and seed C. elegans culture plates (NG-Agar)
Make platinum wire pick for handling worms
Propagate worm cultures by bulk transfer
Transfer single/few worm(s) without poking holes in the agar
Recognize males and hermaphrodites
Distinguish between egg, larval, and adult stages
Recognize L4 larval stage hermaphrodites (based on vulval invagination)
Recognize fresh young hermaphrodites (empty uterus, no fertilized eggs) and gravid hermaphrodites
Mount worms on glass slides for Nomarski observations
Learn to setup a simple genetic cross
Cross Unc/Dpy hermaphrodites to wildtype males
Clone 3 wildtype-looking worms in the F1 generation
Recover the homozygous mutant in F2 generation
Learn to operate the Nomarski microscopes. Read microscopy books and ask the supervisor to demonstrate the correct method of handling.
Cover the Nomarski microscopes after every use. Microscope lenses are expensive and dust can cause damage.
Organize captured images in a folder on the computer. Label files and folders clearly so these could be easily identified. Unorganized files may get deleted without prior warning!
Use the Log Book. It is a MUST for everyone. Also, keep an eye on others to make sure that they are doing it as well.
Report problems with microscopes to supervisor PROMPTLY. It is a collective responsibility of lab workers to keep things running smoothly.
BE A RESPONSIBLE PERSON! DO NOT MISUSE EQUIPMENT/SUPPLIES AND REPORT PROBLEMS PROMPTLY.
NO FOOD OR DRINK IS ALLOWED IN THE LAB!
orientation.txt · Last modified: 2018/05/09 15:38 by admin